Anti-AZI2 antibody [EPR14698] - C-terminal

• Supplier: Abcam
• Catalog: ab192253
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14698
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human AZI2 aa 250 to the C-terminus (C terminal)
• Description: Rabbit Monoclonal
• Gene: AZI2
• Conjugate: Unconjugated

Product images

Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/5000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling AZI2 with ab192253 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) ab150078 was used as the secondary at 1/500 dilution.  Couunterstain DAPI. Negative controls: anti-AZI2 at 1/100 dilution, Secondary ab Goat anti mouse IgG (Alexa Fluor®488 at 1/200 dilution.

Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/1000 dilution + 293 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/1000 dilutionLane 1 : Rat kidney lysateLane 2 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution