All lanes : Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/10000 dilutionLane 1 : HUVEC cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/2000 dilution + Human spleen lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Azurocidin using ab181989 at a 1/40 dilution, or rabbit monoclonal IgG as isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.