All lanes : Anti-B7 DC antibody [EPR1163(2)] (ab187662) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : HepG2 cell lysateLane 3 : THP-1 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-B7 DC antibody [EPR1163(2)] (ab187662) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution