Anti-Bad (phospho S112) antibody [EPR1891(2)]

• Supplier: Abcam
• Catalog: ab129192
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1891(2)
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bad
• Blocking Peptide: Bad peptide
• Description: Rabbit Monoclonal
• Gene: BAD
• Conjugate: Unconjugated

Product images

All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)Lane 1 : Untreated C6 cellsLane 2 : C6 cells treated with Calcyculin ALysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)Lane 1 : Untreated HeLa cellsLane 2 : HeLa cells treated with Calcyculin ALysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilutionLane 1 : HeLa cell lysates, untreatedLane 2 : HeLa cell lysates treated with Calyculin A.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Conditional transgenic expression of PIM1 kinase in prostate induces - Conditional transgenic expression of PIM1 kinase in prostate induces

Narlik-Grassow M, Blanco-Aparicio C, Cecilia Y, Perez M, Munoz-Galvan S, Canamero M, Renner O, Carnero A. PLoS One. 2013;8(4):e60277.