All lanes : Anti-BAF53A antibody [N336B/83] (ab174941) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 5 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutionPerformed under reducing conditions.