Anti-BAF53A antibody - ChIP Grade
| Name | Anti-BAF53A antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab3882 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP ICC/IF ICC/IF WB IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A |
| Blocking Peptide | Human BAF53A peptide |
| Description | Rabbit Polyclonal |
| Gene | ACTL6A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1/1000 dilutionLane 1 : TEV elution of Tip60-TAP clone 1 Lane 2 : TEV elution of Tip60-TAP clone 2Lane 3 : Total extract of Tip60-TAP clone 1Lane 4 : Total extract of Tip60-TAP clone 2Performed under reducing conditions.
Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3882 to BAF53A and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 8 µl of ab3882 and 2x106 cells were used in each ChIP experiment.
ab3882 at a 1/200 dilution staining asynchronous HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes at room temperature. Bound antibody was detected using a Cy3 conjugated Goat anti-rabbit antibody. Nuclei were visualised using DAPI stain. ab3882 gives a predominantly nuclear staining pattern. This image is courtesy of an Abreview submitted by Kirk McManus.See Abreview
All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissueLane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlas ab3882 staining BAF53A in human ovary, showing a distinct and strong staining pattern of the nucleus of the ovarian stromal cells. Paraffin-embedded ovary tissue was incubated with ab3882 (1/35) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab3882 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Product References
Analysis of the SWI/SNF chromatin-remodeling complex during early heart - Analysis of the SWI/SNF chromatin-remodeling complex during early heart
Singh AP, Archer TK. Nucleic Acids Res. 2014 Mar;42(5):2958-75.
Failure to downregulate the BAF53a subunit of the SWI/SNF chromatin remodeling - Failure to downregulate the BAF53a subunit of the SWI/SNF chromatin remodeling
Taulli R, Foglizzo V, Morena D, Coda DM, Ala U, Bersani F, Maestro N, Ponzetto C. Oncogene. 2014 May 1;33(18):2354-62.
Locus-specific proteomics by TChP: targeted chromatin purification. - Locus-specific proteomics by TChP: targeted chromatin purification.
Pourfarzad F, Aghajanirefah A, de Boer E, Ten Have S, Bryn van Dijk T, Kheradmandkia S, Stadhouders R, Thongjuea S, Soler E, Gillemans N, von Lindern M, Demmers J, Philipsen S, Grosveld F. Cell Rep. 2013 Aug 15;4(3):589-600.
Nuclear actin polymerization is required for transcriptional reprogramming of - Nuclear actin polymerization is required for transcriptional reprogramming of
Miyamoto K, Pasque V, Jullien J, Gurdon JB. Genes Dev. 2011 May 1;25(9):946-58.
SWI/SNF chromatin remodeling complex is critical for the expression of - SWI/SNF chromatin remodeling complex is critical for the expression of
Vachtenheim J, Ondrusova L, Borovansky J. Biochem Biophys Res Commun. 2010 Feb 12;392(3):454-9. doi: