Anti-BAFF antibody [Buffy 2]
| Name | Anti-BAFF antibody [Buffy 2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16081 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | Buffy 2 |
| Applications | WB IHC-F FC IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant soluble protein, corresponding to amino acids 83 - 285 of Human BAFF |
| Description | Rat Monoclonal |
| Gene | TNFSF13B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using ab16081.Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
ICC/IF image of ab16081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16081, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-BAFF antibody [Buffy 2] (ab16081) at 1 µg/ml + Human BAFF full length protein (ab78840) at 0.01 µgSecondaryHRP conjugated Rabbit anti-Rat IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/12267_BAFF-Primary-antibodies-ab16081-6.jpg)
Anti-BAFF antibody [Buffy 2] (ab16081) at 1 µg/ml + Human BAFF full length protein (ab78840) at 0.01 µgSecondaryHRP conjugated Rabbit anti-Rat IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes
Overlay histogram showing THP1 cells stained with ab16081 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16081, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgM (mu chain) (ab98368) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgM (ab35768, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
B Lymphocyte Stimulator (BLyS) is expressed in human adipocytes in vivo and is - B Lymphocyte Stimulator (BLyS) is expressed in human adipocytes in vivo and is
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Lichen planopilaris and pseudopelade of Brocq involve distinct disease associated - Lichen planopilaris and pseudopelade of Brocq involve distinct disease associated
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Local BAFF gene silencing suppresses Th17-cell generation and ameliorates - Local BAFF gene silencing suppresses Th17-cell generation and ameliorates
Lai Kwan Lam Q, King Hung Ko O, Zheng BJ, Lu L. Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14993-8. doi:
BAFF production by antigen-presenting cells provides T cell co-stimulation. - BAFF production by antigen-presenting cells provides T cell co-stimulation.
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Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth - Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth
Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF. Blood. 2004 Jan 15;103(2):689-94. Epub 2003 Sep 25.
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and - Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and
He B, Chadburn A, Jou E, Schattner EJ, Knowles DM, Cerutti A. J Immunol. 2004 Mar 1;172(5):3268-79.
Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation - Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation
Novak AJ, Grote DM, Stenson M, Ziesmer SC, Witzig TE, Habermann TM, Harder B, Ristow KM, Bram RJ, Jelinek DF, Gross JA, Ansell SM. Blood. 2004 Oct 15;104(8):2247-53. Epub 2004 Jul 13.