Anti-BAFF antibody [Buffy 2] (FITC)
| Name | Anti-BAFF antibody [Buffy 2] (FITC) |
|---|---|
| Supplier | Abcam |
| Catalog | ab16082 |
| Prices | $189.00 |
| Sizes | 50 tests |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | Buffy 2 |
| Applications | IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant soluble protein, corresponding to amino acids 83 - 285 of Human BAFF |
| Description | Rat Monoclonal |
| Gene | TNFSF13B |
| Conjugate | FITC |
| Supplier Page | Shop |
Product images
Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels staining positive for BAFF using ab16082.Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
Ab16082 detects membrane-bound human BAFF by FACS.Method: HEK 293T cells (5 x 10e5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of ab16082. After washing, cells were analyzed by flow cytometry.
Product References
BAFF production by antigen-presenting cells provides T cell co-stimulation. - BAFF production by antigen-presenting cells provides T cell co-stimulation.
Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, Roosnek E, Tschopp J, Schneider P, French LE. Int Immunol. 2004 Mar;16(3):467-75.
Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation - Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation
Novak AJ, Grote DM, Stenson M, Ziesmer SC, Witzig TE, Habermann TM, Harder B, Ristow KM, Bram RJ, Jelinek DF, Gross JA, Ansell SM. Blood. 2004 Oct 15;104(8):2247-53. Epub 2004 Jul 13.
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and - Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and
He B, Chadburn A, Jou E, Schattner EJ, Knowles DM, Cerutti A. J Immunol. 2004 Mar 1;172(5):3268-79.
Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth - Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth
Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF. Blood. 2004 Jan 15;103(2):689-94. Epub 2003 Sep 25.
Association of BAFF/BLyS overexpression and altered B cell differentiation with - Association of BAFF/BLyS overexpression and altered B cell differentiation with
Groom J, Kalled SL, Cutler AH, Olson C, Woodcock SA, Schneider P, Tschopp J, Cachero TG, Batten M, Wheway J, Mauri D, Cavill D, Gordon TP, Mackay CR, Mackay F. J Clin Invest. 2002 Jan;109(1):59-68.