Anti-Bag1 antibody
| Name | Anti-Bag1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7976 |
| Prices | $385.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Mouse) (C terminal) |
| Description | Rabbit Polyclonal |
| Gene | BAG1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab7976 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7976, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab7976 staining human normal liver. Staining is localised to the nucleus and cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-Bag1 antibody (ab7976) at 1 µg/ml + Human Bag1 full length protein (ab93929) at 0.001 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds
Product References
Identification of internal ribosome entry segment (IRES)-trans-acting factors for - Identification of internal ribosome entry segment (IRES)-trans-acting factors for
Cobbold LC, Spriggs KA, Haines SJ, Dobbyn HC, Hayes C, de Moor CH, Lilley KS, Bushell M, Willis AE. Mol Cell Biol. 2008 Jan;28(1):40-9. Epub 2007 Oct 29.
CRAF autophosphorylation of serine 621 is required to prevent its - CRAF autophosphorylation of serine 621 is required to prevent its
Noble C, Mercer K, Hussain J, Carragher L, Giblett S, Hayward R, Patterson C, Marais R, Pritchard CA. Mol Cell. 2008 Sep 26;31(6):862-72.