BAG1

Gene Symbol BAG1
Entrez Gene 573
Alt Symbol BAG-1, HAP, RAP46
Species Human
Gene Type protein-coding
Description BCL2-associated athanogene
Other Description BAG family molecular chaperone regulator 1|Bcl-2 associating athanogene-1 protein|Bcl-2-binding protein|glucocortoid receptor-associated protein RAP46|receptor-associated protein, 46-KD
Swissprots Q53H32 Q14414 Q9Y2V4 Q5VZF0 Q5VZE8 O75315 Q99933 Q96TG2 Q5VZE9
Accessions EAW58514 EAW58515 EAW58516 EAW58517 Q99933 AF022224 AAC34258 AF116273 AAD25045 AK093238 AK096038 AK222749 BAD96469 AK316577 AM392568 CAL37446 BC001936 AAH01936 BC014774 AAH14774 BG723775 CN478628 U46917 AAD11467 Z35491 CAA84624 NM_001172415 NP_001165886 NM_004323 NP_004314
Function Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. {ECO:0000269|PubMed:12724406, ECO:0000269|PubMed:9305631, ECO:0000269|PubMed:9873016}.
Subcellular Location Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
Tissue Specificity Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). {ECO:0000269|PubMed:9679980}.
Top Pathways Protein processing in endoplasmic reticulum

Bag1 (3.10G3E2) Mouse mAb - 3920 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3.10G3E2
Western blot analysis of extracts from various cell lines using Bag1 (3.10G3E2) Mouse mAb.

Bag-1 (E-11) - sc-376848 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (E-11): sc-376848. Western blot analysis of Bag-1 expression in non-transfected 293T: sc-117752 (A), human Bag-1 transfected 293T: sc-112723 (B) and HeLa (C) whole cell lysates.

Bag-1 (F-7) - sc-25296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (F-7): sc-25296. Western blot analysis of Bag-1 isoform expression in HeLa (A) and Jurkat (B) whole cell lysates.

Bag-1 (E-2) - sc-377454 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (E-2): sc-377454. Western blot analysis of Bag-1 expression in non-transfected 293T: sc-117752 (A), human Bag-1 transfected 293T: sc-112723 (B) and HeLa (C) whole cell lysates.

Bag-1 (A-4) - sc-377419 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (A-4): sc-377419. Western blot analysis of Bag-1 expression in non-transfected 293T: sc-117752 (A), human Bag-1 transfected 293T: sc-112723 (B) and HeLa (C) whole cell lysates.

Bag-1 (CC9E8) - sc-33704 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (CC9E8): sc-33704. Western blot analysis of Bag-1 expression in HeLa whole cell lysate.

Bag-1 (K-20) - sc-31680 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Bag-1 (K-20): sc-31680. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Bag-1 (3.9F1E11) - sc-56004 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (3.9F1E11): sc-56004. Western blot analysis of Bag-1 expression in LNCaP (A), MCF7 (B) and Jurkat (C) whole cell lysates.

Bag-1 (C-20) - sc-31682 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Bag-1 (C-20): sc-31682. Western blot analysis of human recombinant Bag-1 fusion protein.

Bag-1 (C-16) - sc-939 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Bag-1 (C-16): sc-939. Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic localization.

Bag-1 (3.10G3E2) - sc-56003 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (3.10G3E2): sc-56003. Western blot analysis of Bag-1 expression in HeLa (A), LNCaP (B), MCF7 (C), SK-BR-3 (D) and Jurkat (E) whole cell lysates.

Bag-1 (E-17) - sc-31681 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Bag-1 (E-17): sc-31681. Western blot analysis of human recombinant Bag-1 fusion protein.

Bag-1 (FL-274) - sc-8348 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Bag-1 (FL-274): sc-8348. Western blot analysis of Bag-1 isoform expression in HeLa (A) and HL-60 (B) whole cell lysates and mouse testis extract (C).

Bag-1 (SPM232) - sc-56005 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bag-1 (SPM232): sc-56005. Western blot analysis of Bag-1 expression in HeLa whole cell lysate.

Bag-1 (19) - sc-135844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Bag-1 (19): sc-135844. Western blot analysis of Bag-1 expression in HeLa whole cell lysate.

Anti-Bag1 antibody - ab7976 from Abcam

Prices $385.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab7976 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7976, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Bag1 antibody - ab54817 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Bag1 antibody (ab54817) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-Bag1 antibody - ab112493 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Bag1 antibody (ab112493)Lane 1 : Recombinant Human BAG-1 Protein at 0.01 µgLane 2 : Recombinant Human BAG-1 Protein at 0.005 µgLane 3 : Recombinant Human BAG-1 Protein at 0.0025 µgLane 4 : MCF-7 Whole Cell Lysate

Anti-Bag1 antibody - ab118525 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118525, at 5 µg/ml, staining Bag1 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Bag1 antibody [3.10G3E2] - ab2355 from Abcam

Prices $376.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 3.10G3E2
ab2355 staining human breast carcinoma by IHC-P.

Anti-Bag1 antibody [Y166] - ab32109 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y166
Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate

Anti-Bag1 antibody - Aminoterminal end - ab45411 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-Bag1 antibody - Aminoterminal end (ab45411) at 1 µg/mlLane 2 : Anti-Bag1 antibody - Aminoterminal end (ab45411) at 2 µg/mlLane 1 : PC-3 cell lysateLane 2 : PC-3 cell lysate

Anti-BAG1 / BAG-1 Antibody (aa258-285) - LS-C156728 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human brain tissue reacted with BAG1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-BAG1 / BAG-1 Antibody (C-Terminus) IHC-plus™ - LS-B1331 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BAG1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1331 concentration 5 ug/ml.

Anti-BAG1 / BAG-1 Antibody (clone 4A2) - LS-C343626 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4A2
Western blot: Lane 1 Bag-1 (recombinant), lane 2 Jurkat cell lysate; lane 3 U937 cell lysate, lane 4 NIH3T3 cell lysate and lane 5 PC12 cell lysate probed with Bag-1monoclonal antibody (4A2).
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