Anti-BANF1 antibody
| Name | Anti-BANF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56517 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein: MTTSQKHRDF VAEPMGEKPV GSLAGIGEVL GKKLEERGFD KAYVVLGQFL VLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL, corresponding to amino acids 1-90 of Human BANF1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | BANF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
BANF1 antibody (ab56517) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus.
ICC/IF image of ab56517 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56517, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Indications for cell stress in response to adenoviral and baculoviral gene - Indications for cell stress in response to adenoviral and baculoviral gene
Gerner C, Haudek-Prinz VJ, Lackner A, Losert A, Peter-Vorosmarty B, Lorenz O, Grusch M. Electrophoresis. 2010 Jun;31(11):1822-32.
Dynamic host energetics and cytoskeletal proteomes in human immunodeficiency - Dynamic host energetics and cytoskeletal proteomes in human immunodeficiency
Chan EY, Sutton JN, Jacobs JM, Bondarenko A, Smith RD, Katze MG. J Virol. 2009 Sep;83(18):9283-95.