BANF1

Gene Symbol BANF1
Entrez Gene 8815
Alt Symbol BAF, BCRP1, D14S1460, NGPS
Species Human
Gene Type protein-coding
Description barrier to autointegration factor 1
Other Description barrier-to-autointegration factor|breakpoint cluster region protein 1
Swissprots O75531 Q6FGG7 O60558
Accessions EAW74483 EAW74484 EAW74485 EAW74486 O75531 AF044773 AAC08964 AF068235 AAD15901 AF070447 AAC23575 AK311961 BAG34901 BC005942 AAH05942 BC008705 BC107702 AAI07703 BG748852 BU940708 CR542140 CAG46937 NM_001143985 NP_001137457 NM_003860 NP_003851
Function Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. {ECO:0000269|PubMed:11005805, ECO:0000269|PubMed:12163470, ECO:0000269|PubMed:16680152}.
Subcellular Location Nucleus {ECO:0000269|PubMed:16495336}. Cytoplasm {ECO:0000269|PubMed:16495336}. Chromosome {ECO:0000269|PubMed:16495336}. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization.
Tissue Specificity Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.

BAF (A-11) - sc-166324 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BAF (A-11): sc-166324. Western blot analysis of BAF expression in Jurkat whole cell lysate.

BAF (N-16) - sc-27836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BAF (FL-89) - sc-33787 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BAF (FL-89): sc-33787. Western blot analysis of BAF expression in HeLa nuclear extract.

Anti-BANF1 antibody - ab56517 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
BANF1 antibody (ab56517) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus.

Anti-BANF1 antibody - ab115226 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human heart (formalin fixed, paraffin embedded) stained with ab115226 at 5 ug/ml followed by biotinylated Goat anti Rabbit IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

Anti-BANF1 antibody [EPR7668] - ab129184 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7668
All lanes : Anti-BANF1 antibody [EPR7668] (ab129184) at 1/1000 dilutionLane 1 : HeLa cell lysatesLane 2 : HepG2 cell lysatesLane 3 : 293T cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-BANF1 antibody [EPR7669] - ab129074 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7669
Overlay histogram showing HepG2 cells stained with ab129074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129074, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-BANF1 / BAF / BCRP1 Antibody - LS-C125162 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65, APC) - LS-C252380 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65, AP) - LS-C252382 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65, Biotin) - LS-C252381 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65, FITC) - LS-C252383 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (C-Terminus) - LS-C151667 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
BANF1 / BAF / BCRP1 antibody Western Blot of MCF7.

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65, Azide-free, HRP) - LS-C252384 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65, PE) - LS-C252387 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (aa36-65) - LS-C329253 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BANF1 / BAF / BCRP1 Antibody (clone 3F10-4G12) IHC-plus™ - LS-B4474 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3F10-4G12
Anti-BANF1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4474 concentration 5 ug/ml.

Anti-BANF1 / BAF / BCRP1 Antibody (aa37-65) - LS-C156734 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
BANF1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-BANF1 / BAF / BCRP1 Antibody (Internal, clone EPR7668) - LS-C154412 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7668
IHC of paraffin-embedded human colonic carcinoma tissue using anti-BANF1 antibody.

Anti-BANF1 / BAF / BCRP1 Antibody (C-Terminus) IHC-plus™ - LS-B447 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BANF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B447 concentration 5 ug/ml.

Anti-BANF1 / BAF / BCRP1 Antibody (Internal, clone EPR7669) IHC-plus™ - LS-B9368 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7669
Anti-BANF1 / BAF / BCRP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-BANF1 / BAF / BCRP1 Antibody (C-Terminus) IHC-plus™ - LS-B1332 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BANF1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1332 concentration 5 ug/ml.

BANF1 Antibody (3F10-4G12) - H00008815-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F10-4G12
Western Blot: BANF1 Antibody (3F10-4G12) [H00008815-M01] - BANF1 monoclonal antibody (M01), clone 3F10-4G12 Analysis of BANF1 expression in Jurkat.

BANF1 Antibody - NBP2-38442 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: BANF1 Antibody [NBP2-38442] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

BANF1 Antibody - NBP1-76751 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: BANF1 Antibody [NBP1-76751] - Analysis in Hela cell lysate with antibody at 1 ug/mL.
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