Anti-BANF1 antibody [EPR7668]

• Supplier: Abcam
• Catalog: ab129184
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7668
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Dog, Human
• Antigen: Synthetic peptide corresponding to residues in Human BANF1 (O75531)
• Description: Rabbit Monoclonal
• Gene: BANF1
• Conjugate: Unconjugated

Product images

All lanes : Anti-BANF1 antibody [EPR7668] (ab129184) at 1/1000 dilutionLane 1 : HeLa cell lysatesLane 2 : HepG2 cell lysatesLane 3 : 293T cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab129184 staining BANF1 in MDCK cell pellets in paraffin by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cell pellets were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris/EDTA pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

ab129184, at a 1/100 dilution, staining BANF1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemistry.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cells)cells labeling BANF1 with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling BANF1 with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with weakly cytoplasmic staining on Hela cell line. The nuclear counterstain is DAPI (blue).

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD