Overlay histogram showing HepG2 cells stained with ab129074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129074, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-BANF1 antibody [EPR7669] (ab129074) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/12463_BANF1-Primary-antibodies-ab129074-1.jpg)
All lanes : Anti-BANF1 antibody [EPR7669] (ab129074) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
ab129074, at 1/100 dilution, staining BANF1 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.