Anti-Bassoon antibody
| Name | Anti-Bassoon antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76065 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide from internal part of human Bassoon conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | BSN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab76065 (1µg/ml) staining bassoon in human cerebellum (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.Insert depicts negative control (no primary antibody).Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab76065 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of frozen mouse retinal sections, detecting synaptic contacts established by photoreceptors in the outer plexiform layer, by staining Bassoon (red) with ab76065. Sections were fixed in 4% paraformaldehyde before incubating with primary antibody (1/1000). An AlexaFluor®568-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
Product References
Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal - Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal
Khan M, Goldsmith CR, Huang Z, Georgiou J, Luyben TT, Roder JC, Lippard SJ, Okamoto K. Proc Natl Acad Sci U S A. 2014 May 6;111(18):6786-91. doi:
Environmental enrichment extends photoreceptor survival and visual function in a - Environmental enrichment extends photoreceptor survival and visual function in a
Barone I, Novelli E, Piano I, Gargini C, Strettoi E. PLoS One. 2012;7(11):e50726.