BSN

Gene Symbol BSN
Entrez Gene 8927
Alt Symbol ZNF231
Species Human
Gene Type protein-coding
Description bassoon presynaptic cytomatrix protein
Other Description bassoon (presynaptic cytomatrix protein)|neuronal double zinc finger protein|protein bassoon|zinc finger protein 231
Swissprots Q9UPA5 O43161 Q7LGH3
Accessions CAA77176 EAW64992 EAW64993 Q9UPA5 AB007894 BAA23707 AF052224 AAC83555 AW131629 NM_003458 NP_003449
Function Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cytoplasm, cytoskeleton. Note=Localized to the active zone of presynaptic density. {ECO:0000250}.
Tissue Specificity Exclusively expressed in brain. {ECO:0000269|PubMed:9806829}.

Bassoon (D63B6) Rabbit mAb - 6897 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D63B6
Western blot analysis of extracts from mouse and rat brain tissues using Bassoon (D63B6) Rabbit mAb.

bassoon (C-15) - sc-18566 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

bassoon (E-20) - sc-18565 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Bassoon antibody - ab76065 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab76065 (1µg/ml) staining bassoon in human cerebellum (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.Insert depicts negative control (no primary antibody).Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Bassoon antibody - ab101772 from Abcam

Host Goat
Clonality Polyclonal
ICC/IF image of ab101772 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101772, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Bassoon antibody - ab110426 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Bassoon antibody (ab110426) at 1/1000 dilutionLane 1 : Rat brain lysateLane 2 : Mouse brain lysateLysates/proteins at 10 µg per lane.

Anti-BSN / Bassoon Antibody (aa738-1035, clone SAP7F407) - LS-C74651 from LifeSpan Bioscience

Prices $495.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone SAP7F407

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 390) - LS-C232163 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041) - LS-C182557 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 488) - LS-C232165 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 565) - LS-C232166 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 594) - LS-C232167 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 633) - LS-C232168 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 655) - LS-C232169 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 680) - LS-C232170 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Atto 700) - LS-C232171 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, AP) - LS-C232172 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody - LS-C150464 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of Bassoon in mouse and rat brain lysate using a 1:1000 dilution of BSN / Bassoon antibody.

Anti-BSN / Bassoon Antibody (aa786-1041, APC) - LS-C232173 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, Biotin) - LS-C232174 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, FITC) - LS-C232175 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, PE, Atto 594) - LS-C232177 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, PerCP) - LS-C232178 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, SA) - LS-C232180 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BSN / Bassoon Antibody (aa786-1041, RPE) - LS-C232179 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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