Anti-BAT3 antibody

• Supplier: Abcam
• Catalog: ab88292
• Prices: $385.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Human
• Antigen: Full length Human BAT3 (NP_542433
• Description: Mouse Polyclonal
• Gene: BAG6
• Conjugate: Unconjugated

Product images

Anti-BAT3 antibody (ab88292) at 1 µg/ml + A-431 cell lysate with BAT3 expression at 50 µgSecondaryGoat Anti-Mouse IgG at 1/2500 dilution

All lanes : Anti-BAT3 antibody (ab88292) at 1 µg/mlLane 1 : BAT3 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG at 1/2500 dilution

Immunofluorescence of ab88292 on HeLa cell with antibody concentration at 10 ug/ml

IHC image of ab88292 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88292, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Scythe cleavage during Fas (APO-1)-and staurosporine-mediated apoptosis. - Scythe cleavage during Fas (APO-1)-and staurosporine-mediated apoptosis.

Preta G, Fadeel B. FEBS Lett. 2012 Mar 23;586(6):747-52.

BAT3 guides misfolded glycoproteins out of the endoplasmic reticulum. - BAT3 guides misfolded glycoproteins out of the endoplasmic reticulum.

Claessen JH, Ploegh HL. PLoS One. 2011;6(12):e28542.