Anti-Bax antibody
| Name | Anti-Bax antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53154 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Bax |
| Description | Rabbit Polyclonal |
| Gene | BAX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence analysis of A549 cells, using ab53154 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.
All lanes : Anti-Bax antibody (ab53154) at 1/500 dilutionLane 1 : Extracts from 293 cells, untreatedLane 2 : Extracts from 293 cells, treated with the immunizing peptide
Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using ab53154 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.
Ab53154 staining human normal gallbladder tissue. Staining is localised to cell membrane and cytoplasm.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
ELISA analysis of primary cultured rat cortical neurons, detecting Bax using ab53154.Cells were treated with (Tat) or without Tat (control) and either Estradiol, Genistein or Daidzein. Lysates were prepared after 24 hours. A 96-well plate was coated using carbonate coating buffer. 20 µg cell lysate was added to wells and incubated overnight at 4°C. Plates were blocked with 1% BSA for 2 hr at room temperature. Primary antibody (1/5000) was added to sample wells before incubating overnight at 4°C. A goat anti-rabbit ALP-conjugated IgG was used as secondary antibody and phosphatase substrate mixture was added. Absorbance was read at 650nm.
Product References
Soy isoflavones genistein and daidzein exert anti-apoptotic actions via a - Soy isoflavones genistein and daidzein exert anti-apoptotic actions via a
Adams SM, Aksenova MV, Aksenov MY, Mactutus CF, Booze RM. PLoS One. 2012;7(5):e37540.
ER-beta mediates 17beta-estradiol attenuation of HIV-1 Tat-induced apoptotic - ER-beta mediates 17beta-estradiol attenuation of HIV-1 Tat-induced apoptotic
Adams SM, Aksenova MV, Aksenov MY, Mactutus CF, Booze RM. Synapse. 2010 Nov;64(11):829-38.