Anti-Bax antibody [E63]
| Name | Anti-Bax antibody [E63] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32503 |
| Prices | $404.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E63 |
| Applications | WB IHC-P IP FC ELISA |
| Species Reactivities | Mouse, Rat, Human, Bovine |
| Antigen | Synthetic peptide within Human Bax aa 1-100 (N terminal) |
| Blocking Peptide | Bax peptide |
| Description | Rabbit Monoclonal |
| Gene | BAX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Bax antibody [E63] (ab32503) at 1/2000 dilution + HeLa cell lysateSecondaryGoat anti-rabbit HRP antibodydeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/12647_ab32503-208332-ab32503.jpg)
Anti-Bax antibody [E63] (ab32503) at 1/2000 dilution + HeLa cell lysateSecondaryGoat anti-rabbit HRP antibodydeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human lymph node using anti-Bax Rabbit Monoclonal Antibody (ab32503) at 1/250 dilution.
Overlay histogram showing HeLa cells stained with ab32503 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32503, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Standard Curve for Bax (Analyte: ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/12650_Bax-Primary-antibodies-ab32503-3.jpg)
Standard Curve for Bax (Analyte: ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.
Product References
The antiproliferative effect of C2-ceramide on lung cancer cells through - The antiproliferative effect of C2-ceramide on lung cancer cells through
Lin IL, Chou HL, Lee JC, Chen FW, Fong Y, Chang WC, Huang HW, Wu CY, Chang WT, Wang HM, Chiu CC. Cancer Cell Int. 2014 Jan 6;14(1):1.
Amelioration of aspirin induced oxidative impairment and apoptotic cell death by - Amelioration of aspirin induced oxidative impairment and apoptotic cell death by
Bhattacharyya S, Ghosh S, Sil PC. PLoS One. 2014 Feb 19;9(2):e89026.
Mangiferin has an additive effect on the apoptotic properties of hesperidin in - Mangiferin has an additive effect on the apoptotic properties of hesperidin in
Bartoszewski R, Hering A, Marszall M, Stefanowicz Hajduk J, Bartoszewska S, Kapoor N, Kochan K, Ochocka R. PLoS One. 2014 Mar 14;9(3):e92128.
Up-regulation of eEF1A2 promotes proliferation and inhibits apoptosis in prostate - Up-regulation of eEF1A2 promotes proliferation and inhibits apoptosis in prostate
Sun Y, Du C, Wang B, Zhang Y, Liu X, Ren G. Biochem Biophys Res Commun. 2014 Jul 18;450(1):1-6. doi:
13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3 - 13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3
Cui SX, Zhang HL, Xu WF, Qu XJ. Eur J Pharmacol. 2014 Jul 5;734:50-9.
Protective role of peroxisome proliferator-activated receptor beta/delta in acute - Protective role of peroxisome proliferator-activated receptor beta/delta in acute
Bao XC, Fang YQ, You P, Zhang S, Ma J. Respir Physiol Neurobiol. 2014 Aug 1;199:9-18.
Activation of PI3K/Akt pathway limits JNK-mediated apoptosis during EV71 - Activation of PI3K/Akt pathway limits JNK-mediated apoptosis during EV71
Zhang H, Li F, Pan Z, Wu Z, Wang Y, Cui Y. Virus Res. 2014 Nov 4;192:74-84.
Activation of histamine H4 receptor inhibits TNFalpha/IMD-0354-induced apoptosis - Activation of histamine H4 receptor inhibits TNFalpha/IMD-0354-induced apoptosis
Stegajev V, Kouri VP, Salem A, Rozov S, Stark H, Nordstrom DC, Konttinen YT. Apoptosis. 2014 Dec;19(12):1702-11.
The anti-apoptotic effect of hydrogen sulfide attenuates injuries to the - The anti-apoptotic effect of hydrogen sulfide attenuates injuries to the
Nie L, Hu Y, Yan X, Li M, Chen L, Li H, Li X, Zhou H, Zheng Y. Respir Physiol Neurobiol. 2013 Aug 1;188(1):29-38. doi:
RECK regulated endoplasmic reticulum stress response and enhanced - RECK regulated endoplasmic reticulum stress response and enhanced
Chen Y, Tsai YH, Tseng SH. Surgery. 2013 Nov;154(5):968-79.