Lanes 1 - 2 : anti-p130 CasLanes 3 - 4 : Anti-BCAR1 (phospho Y751) antibody (ab45486) at 1/2000 dilutionLanes 5 - 6 : anti-p130 Cas (Tyr-762)Lane 1 : human endothelial cells serum starved overnightLane 2 : human endothelial cells treated with pervanadate (1 mM) for 30 minutesLane 3 : human endothelial cells serum starved overnightLane 4 : human endothelial cells treated with pervanadate (1 mM) for 30 minutesLane 5 : human endothelial cells serum starved overnightLane 6 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes