Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/mlLane 1 : Whole cell lysate from MCF7 cells at 50 µgLane 2 : Whole cell lysate from MCF7 cells at 15 µgLane 3 : Whole cell lysate from MCF7 cells at 5 µgdeveloped using the ECL technique
Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3. Lane 1: ab71162 at 3µg/mg whole cell lysate. Lane 2: Control IgG. All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 1 second.