All lanes : Anti-Bcl6 antibody (ab86861) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Bcl6 antibody (ab86861) at 1 µg/mlLane 1 : Brain (Mouse) Tissue Lysate (ab27253)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)Lane 3 : Brain (Rat) Tissue Lysate (ab7942)Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Bcl6 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Bcl6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab86861.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to anti-rabbit HRP (ab99697).Band: 105kDa; Bcl6](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/13182_Bcl6-Primary-antibodies-ab86861-4.jpg)
Bcl6 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Bcl6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab86861.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to anti-rabbit HRP (ab99697).Band: 105kDa; Bcl6