Anti-Beclin 1 antibody

• Supplier: Abcam
• Catalog: ab62472
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P IHC-F ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide as a part of human, rat, mouse Beclin 1 conjugated to immunogenic carrier protein
• Description: Rabbit Polyclonal
• Gene: BECN1
• Conjugate: Unconjugated

Product images

ab62472 (1/500) staining Beclin 1 in rat brain tissue sections by IHC-Fr (Immunohistochemistry of frozen sections). Nuclei were counterstained with DAPI.

ICC/IF image of ab62472 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62472, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Dengue virus infection induces autophagy: an in vivo study. - Dengue virus infection induces autophagy: an in vivo study.

Lee YR, Hu HY, Kuo SH, Lei HY, Lin YS, Yeh TM, Liu CC, Liu HS. J Biomed Sci. 2013 Sep 8;20:65.

Inhibition of autophagy contributes to ischemic postconditioning-induced - Inhibition of autophagy contributes to ischemic postconditioning-induced

Gao L, Jiang T, Guo J, Liu Y, Cui G, Gu L, Su L, Zhang Y. PLoS One. 2012;7(9):e46092.