Anti-Beclin 1 antibody [EPR1733Y]

• Supplier: Abcam
• Catalog: ab51031
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1733Y
• Applications: ICC/IF ICC/IF WB IP IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues near the C-terminus of human Beclin 1
• Description: Rabbit Monoclonal
• Gene: BECN1
• Conjugate: Unconjugated

Product images

Anti-Beclin 1 antibody [EPR1733Y] (ab51031) at 1/5000 dilution + A431 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled. at 1/2000 dilution

Ab51031 (1:50) staining human Beclin 1 in human breast tissue by immunohistochemistry using paraffin embedded tissue.

Immunofluorescent staining of HeLa cells using ab51031 (1:50).

ab51031 staining beclin1 in MCF7 cells treated with pterostilbene (ab120961), by ICC/IF. Increase in beclin1 expression correlates with increased concentration of pterostilbene as described in literature.The cells were incubated at 37°C for 48h in media containing different concentrations of ab120961 (pterostilbene) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51031 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Product References

IFN-gamma stimulates autophagy-mediated clearance of Burkholderia cenocepacia in - IFN-gamma stimulates autophagy-mediated clearance of Burkholderia cenocepacia in

Assani K, Tazi MF, Amer AO, Kopp BT. PLoS One. 2014 May 5;9(5):e96681.

Defective autophagy in chondrocytes with Kashin-Beck disease but higher than - Defective autophagy in chondrocytes with Kashin-Beck disease but higher than

Wu C, Zheng J, Yao X, Shan H, Li Y, Xu P, Guo X. Osteoarthritis Cartilage. 2014 Nov;22(11):1936-46. doi:

Histone deacetylase inhibitor potentiated the ability of MTOR inhibitor to induce - Histone deacetylase inhibitor potentiated the ability of MTOR inhibitor to induce

Dong LH, Cheng S, Zheng Z, Wang L, Shen Y, Shen ZX, Chen SJ, Zhao WL. J Hematol Oncol. 2013 Jul 18;6:53.

The residual tumor autophagy marker LC3B serves as a prognostic marker in local - The residual tumor autophagy marker LC3B serves as a prognostic marker in local

Chen S, Jiang YZ, Huang L, Zhou RJ, Yu KD, Liu Y, Shao ZM. Clin Cancer Res. 2013 Dec 15;19(24):6853-62.

Suppression of autophagy exacerbates Mefloquine-mediated cell death. - Suppression of autophagy exacerbates Mefloquine-mediated cell death.

Shin JH, Park SJ, Jo YK, Kim ES, Kang H, Park JH, Lee EH, Cho DH. Neurosci Lett. 2012 May 2;515(2):162-7.

Two-compartment tumor metabolism: autophagy in the tumor microenvironment and - Two-compartment tumor metabolism: autophagy in the tumor microenvironment and

Salem AF, Whitaker-Menezes D, Lin Z, Martinez-Outschoorn UE, Tanowitz HB, Al-Zoubi MS, Howell A, Pestell RG, Sotgia F, Lisanti MP. Cell Cycle. 2012 Jul 1;11(13):2545-56.

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts - Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts

Chiavarina B, Whitaker-Menezes D, Martinez-Outschoorn UE, Witkiewicz AK, Birbe R, Howell A, Pestell RG, Smith J, Daniel R, Sotgia F, Lisanti MP. Cancer Biol Ther. 2011 Dec 15;12(12):1101-13.

Matrix remodeling stimulates stromal autophagy, "fueling" cancer cell - Matrix remodeling stimulates stromal autophagy, "fueling" cancer cell

Castello-Cros R, Bonuccelli G, Molchansky A, Capozza F, Witkiewicz AK, Birbe RC, Howell A, Pestell RG, Whitaker-Menezes D, Sotgia F, Lisanti MP. Cell Cycle. 2011 Jun 15;10(12):2021-34. Epub 2011 Jun 15.

Activation of autophagy in mesenchymal stem cells provides tumor stromal support. - Activation of autophagy in mesenchymal stem cells provides tumor stromal support.

Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. Carcinogenesis. 2011 Jul;32(7):964-72.

The significance of expression of autophagy-related gene Beclin, Bcl-2, and Bax - The significance of expression of autophagy-related gene Beclin, Bcl-2, and Bax

Yao Q, Chen J, Lv Y, Wang T, Zhang J, Fan J, Wang L. Tumour Biol. 2011 Dec;32(6):1163-71.