ab14927 staining HEK-293 cells transfected with human Bestrophin 1 by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% goat serum for 20 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary. Bestrophin transfected in HEK-293 cells is labeled with green fluorescence. Nuclei were counterstained with DAPI (blue).See Abreview
All lanes : Anti-Bestrophin antibody (ab14927) at 1/200 dilutionLane 1 : Whole cell lysate from bovine retinal pigment epithelial cellsLane 2 : Whole cell lysate from non transfected HEK 293 cellsLysates/proteins at 25 µg per lane.SecondaryHRP conjugated goat anti-rabbit at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.