Anti-Bestrophin antibody [E6-6]
| Name | Anti-Bestrophin antibody [E6-6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2182 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | E6-6 |
| Applications | WB IP IHC-F ICC/IF ICC/IF |
| Species Reactivities | Bovine, Dog, Human, Pig, Monkey |
| Antigen | Recombinant fragment: KDHMDPYWALENRDEAHS , corresponding to C terminal amino acids 568-585 of Human Bestrophin |
| Description | Mouse Monoclonal |
| Gene | BEST1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2182 staining pig retinal pigment epithelium tissue sections. The tissue was fixed with paraformaldehyde, permeabilised in 0.5% TX100, 5% goat serum, 1XPBS, and blocked with 5% serum for 20 minutes at 25°C. The primary anitbody was diluted 1/500 in 1% goat serum, 0.1% TX100, 1XPBS and incubated for 12 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-mouse was used as the secondary antibody.The image shows bestrophin, which localises to the retinal pigment epithelial (RPE) cells, labeled with green fluorescence. Nuclei were counterstained with DAPI (blue).See Abreview
![All lanes : Anti-Bestrophin antibody [E6-6] (ab2182) at 1/1000 dilutionLane 1 : Human RPE, retinal pigment epithelial cell lysateLane 2 : Non transfected HEK 293 cell extractLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-mousedeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 67 kDa (why is the actual band size different from the predicted?)Exposure time : 5 minutesThis image is courtesy of an Abreview submitted by Dr Vladimir MilenkovicSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/13444_ab2182_2.jpg)
All lanes : Anti-Bestrophin antibody [E6-6] (ab2182) at 1/1000 dilutionLane 1 : Human RPE, retinal pigment epithelial cell lysateLane 2 : Non transfected HEK 293 cell extractLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-mousedeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 67 kDa (why is the actual band size different from the predicted?)Exposure time : 5 minutesThis image is courtesy of an Abreview submitted by Dr Vladimir MilenkovicSee Abreview
ab2182 staining cells of bovine retinal pigment epithelium (RPE) by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% goat serum for 20 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody was used as the secondary. Image A shows bestrophin localized in the basolateral side of the RPE cells labeled with red fluorescence (nuclei were counterstained with DAPI (blue)). Image B, a phase contrast (DIC) image, shows cellular structure.See Abreview
Western blot detection of Bestrophin (68 kDa)from human RPE cell lysate.
Product References
Effects of alternative splicing on the function of bestrophin-1 calcium-activated - Effects of alternative splicing on the function of bestrophin-1 calcium-activated
Kuo YH, Abdullaev IF, Hyzinski-Garcia MC, Mongin AA. Biochem J. 2014 Mar 15;458(3):575-83.
Tumorigenicity studies of induced pluripotent stem cell (iPSC)-derived retinal - Tumorigenicity studies of induced pluripotent stem cell (iPSC)-derived retinal
Kanemura H, Go MJ, Shikamura M, Nishishita N, Sakai N, Kamao H, Mandai M, Morinaga C, Takahashi M, Kawamata S. PLoS One. 2014 Jan 14;9(1):e85336.
In-depth characterisation of Retinal Pigment Epithelium (RPE) cells derived from - In-depth characterisation of Retinal Pigment Epithelium (RPE) cells derived from
Brandl C, Zimmermann SJ, Milenkovic VM, Rosendahl SM, Grassmann F, Milenkovic A, Hehr U, Federlin M, Wetzel CH, Helbig H, Weber BH. Neuromolecular Med. 2014 Sep;16(3):551-64.
Disease-associated missense mutations in bestrophin-1 affect cellular trafficking - Disease-associated missense mutations in bestrophin-1 affect cellular trafficking
Milenkovic VM, Rohrl E, Weber BH, Strauss O. J Cell Sci. 2011 Sep 1;124(Pt 17):2988-96.
The presence of bestrophin-1 modulates the Ca2+ recruitment from Ca2+ stores in - The presence of bestrophin-1 modulates the Ca2+ recruitment from Ca2+ stores in
Neussert R, Muller C, Milenkovic VM, Strauss O. Pflugers Arch. 2010 Jun;460(1):163-75.
Regulation of the renin expression in the retinal pigment epithelium by systemic - Regulation of the renin expression in the retinal pigment epithelium by systemic
Milenkovic VM, Brockmann M, Meyer C, Desch M, Schweda F, Kurtz A, Todorov V, Strauss O. Am J Physiol Renal Physiol. 2010 Aug;299(2):F396-403. doi:
Bestrophin interacts physically and functionally with protein phosphatase 2A. - Bestrophin interacts physically and functionally with protein phosphatase 2A.
Marmorstein LY, McLaughlin PJ, Stanton JB, Yan L, Crabb JW, Marmorstein AD. J Biol Chem. 2002 Aug 23;277(34):30591-7. Epub 2002 Jun 10.
Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), - Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2),
Marmorstein AD, Marmorstein LY, Rayborn M, Wang X, Hollyfield JG, Petrukhin K. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12758-63.
Bestrophin gene mutations in patients with Best vitelliform macular dystrophy. - Bestrophin gene mutations in patients with Best vitelliform macular dystrophy.
Caldwell GM, Kakuk LE, Griesinger IB, Simpson SA, Nowak NJ, Small KW, Maumenee IH, Rosenfeld PJ, Sieving PA, Shows TB, Ayyagari R. Genomics. 1999 May 15;58(1):98-101.
Identification of the gene responsible for Best macular dystrophy. - Identification of the gene responsible for Best macular dystrophy.
Petrukhin K, Koisti MJ, Bakall B, Li W, Xie G, Marknell T, Sandgren O, Forsman K, Holmgren G, Andreasson S, Vujic M, Bergen AA, McGarty-Dugan V, Figueroa D, Austin CP, Metzker ML, Caskey CT, Wadelius C. Nat Genet. 1998 Jul;19(3):241-7.