Immunofluorescent analysis of HeLa cells using ab77208 at 10 ug/ml.
Capture ELISA using ab77208 and recombinant tagged Beta Arrestin 2.
Anti-Beta Arrestin 2 antibody (ab77208) at 1/500 dilution + recombinant tagged Beta Arrestin 2 (the immunogen) at 0.2 µgSecondarygoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilutiondeveloped using the ECL techniqueObserved band size : 38 kDa (why is the actual band size different from the predicted?)
Anti-Beta Arrestin 2 antibody (ab77208) at 1/500 dilution + HeLa cell lysate at 50 µgSecondarygoat anti-mouse (H&L)-HRP conjugate at 1/2500 dilutiondeveloped using the ECL techniqueObserved band size : 46 kDa (why is the actual band size different from the predicted?)