Anti-beta III Tubulin antibody

• Supplier: Abcam
• Catalog: ab18207
• Prices: $401.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F FC IHC-P IHC-F ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human, Pig, Marmoset
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human neuron specific beta III Tubulin
• Blocking Peptide: Human beta III Tubulin peptide
• Description: Rabbit Polyclonal
• Gene: TUBB3
• Conjugate: Unconjugated

Product images

ab18207 staining beta III tubulin in PC-12 cells treated with venlafaxine hydrochloride (ab120715), by ICC/IF. Increase in the number and length of neurites (stained with beta III tubulin) correlates with increased concentration of venlafaxine hydrochloride, as described in literature.The NGF treated cells were incubated at 37°C for 6 hour in media containing different concentrations of ab120715 (venlafaxine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18207 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207) on Dogfish/Catshark Tissue sections (head: snout region). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/2000 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).

All lanes : Anti-beta III Tubulin antibody (ab18207) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with Human beta III Tubulin peptide (ab18660) at 2 µg/mlLane 5 : Brain (Mouse) Tissue Lysate with Human beta III Tubulin peptide (ab18660) at 2 µg/mlLane 6 : Brain (Rat) Tissue Lysate with Human beta III Tubulin peptide (ab18660) at 2 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

All lanes : Anti-beta III Tubulin antibody (ab18207) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab18207 stained SH-SY-5Y cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18207, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

ab18207 at 1/2000 staining rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.See Abreview

ab18207 at 1/2000 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and an enzymatic antigen retrieval step was performed prior to incubation with the antibody for 16 hours. A biotinylated goat anti-rabbit IgG was used as the secondary.See Abreview

ab18207 staining rat somatosensory cortex tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in TritonX100 prior to blocking in 3% BSA for 1 hour at RT.  The primary antibody was diluted 1/1000 and incubated with the sample for 18 hrs at 4°C.  A biotinylated goat anti-rabbit IgG was used as the secondary antibody. The anti-beta III tubulin staining is clearly visible in dendrites of pyramidal cells passing from layer V to the superficial layers.  (Magnification = 200x)See Abreview

ab18207 staining human cultured stem cells differentiated to neurons, by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X prior to blocking with 1% BSA for 30 minutes at 27°C.  The primary antibody was used undiluted and incubated with the sample for 24 hours at 4°C.  A PE-conjugated rabbit anti-rabbit antibody was used as the secondary.See Abreview

Product References

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Pedroni SM, Gonzalez JM, Wade J, Jansen MA, Serio A, Marshall I, Lennen RJ, Girardi G. Biochim Biophys Acta. 2014 Jan;1842(1):107-15.

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Wolpert F, Tritschler I, Steinle A, Weller M, Eisele G. Neuro Oncol. 2014 Mar;16(3):382-91.

Diffusion MR Microscopy of Cortical Development in the Mouse Embryo. - Diffusion MR Microscopy of Cortical Development in the Mouse Embryo.

Aggarwal M, Gobius I, Richards LJ, Mori S. Cereb Cortex. 2014 Feb 10.

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Carpanini SM, McKie L, Thomson D, Wright AK, Gordon SL, Roche SL, Handley MT, Morrison H, Brownstein D, Wishart TM, Cousin MA, Gillingwater TH, Aligianis IA, Jackson IJ. Dis Model Mech. 2014 Jun;7(6):711-22.

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Garcia-Gutierrez P, Juarez-Vicente F, Wolgemuth DJ, Garcia-Dominguez M. J Cell Sci. 2014 Jun 1;127(Pt 11):2554-64.

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Smith TH, Ngwainmbi J, Hashimoto A, Dewey WL, Akbarali HI. Physiol Rep. 2014 Sep 4;2(9). pii: e12140.

Intrinsic innate immunity fails to control herpes simplex virus and vesicular - Intrinsic innate immunity fails to control herpes simplex virus and vesicular

Rosato PC, Leib DA. J Virol. 2014 Sep 1;88(17):9991-10001.

CFTR-deficient pigs display peripheral nervous system defects at birth. - CFTR-deficient pigs display peripheral nervous system defects at birth.

Reznikov LR, Dong Q, Chen JH, Moninger TO, Park JM, Zhang Y, Du J, Hildebrand MS, Smith RJ, Randak CO, Stoltz DA, Welsh MJ. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3083-8. doi:

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Alonso A, Reinz E, Leuchs B, Kleinschmidt J, Fatar M, Geers B, Lentacker I, Hennerici MG, de Smedt SC, Meairs S. Mol Ther Nucleic Acids. 2013 Feb 19;2:e73.

Lead induces similar gene expression changes in brains of gestationally exposed - Lead induces similar gene expression changes in brains of gestationally exposed

Sanchez-Martin FJ, Fan Y, Lindquist DM, Xia Y, Puga A. PLoS One. 2013 Nov 19;8(11):e80558.