TUBB3

Gene Symbol TUBB3
Entrez Gene 10381
Alt Symbol CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
Species Human
Gene Type protein-coding
Description tubulin, beta 3 class III
Other Description class III beta-tubulin|tubulin beta-3 chain|tubulin beta-4 chain|tubulin beta-III
Swissprots Q9BTZ0 A8K854 Q9BW10 Q13509
Accessions EAW66669 EAW66674 EAW66675 Q13509 AF427491 AAL28094 AK058140 AK122757 BAG53710 AK127418 AK223039 BAD96759 AK292219 BAF84908 AK307201 AK314616 BAG37182 AU142509 BC000748 AAH00748 BC001678 AAH01678 BC003021 AAH03021 BC047518 AAH47518 BC064975 CD621827 DA054466 S62643 U47634 AAC52035 NM_001197181 NP_001184110 NM_006086 NP_006077
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. {ECO:0000269|PubMed:20074521}.
Subcellular Location Cytoplasm, cytoskeleton.
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. {ECO:0000269|PubMed:14736079, ECO:0000269|PubMed:20191564}.
Top Pathways Gap junction, Pathogenic Escherichia coli infection, Phagosome

β3-Tubulin (D71G9) XP ® Rabbit mAb - 5568 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D71G9
Western blot analysis of HeLa and human cerebellum using β3-Tubulin (D71G9) XP ® Rabbit mAb.

β3-Tubulin (D65A4) XP ® Rabbit mAb - 5666 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D65A4
Western blot analysis of extracts from various cell lines and tissues using β3-Tubulin (D65A4) XP ® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower).

β3-Tubulin (TU-20) Mouse mAb - 4466 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone TU-20
Western blot analysis of extracts from fetal rat and neonatal mouse brain using β3-Tubulin (TU-20) Mouse mAb.

Pan β Tubulin (C-10) - sc-398937 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pan β Tubulin (C-10): sc-398937. Western blot analysis of Pan β Tubulin expression in BJAB (A), Daoy (B) and IMR-32 (C) whole cell lysates.

β3 Tubulin (2G10) - sc-80005 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
β3 Tubulin (2G10): sc-80005. Western blot analysis of β3 Tubulin expression in A-431 (A), K-562 (B) and PC-12 (C) whole cell lysates.

β3 Tubulin (TUJ-1) - sc-58888 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
β3 Tubulin (TUJ-1): sc-58888. Western blot analysis of β3 Tubulin expression in BJAB whole cell lysate.

Anti-beta III Tubulin (phospho S172) antibody - ab76286 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence. Image 1: untreated. Image 2: in the presence of the phospho peptide.

Anti-beta III Tubulin antibody - ab18207 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab18207 staining beta III tubulin in PC-12 cells treated with venlafaxine hydrochloride (ab120715), by ICC/IF. Increase in the number and length of neurites (stained with beta III tubulin) correlates with increased concentration of venlafaxine hydrochloride, as described in literature.The NGF treated cells were incubated at 37°C for 6 hour in media containing different concentrations of ab120715 (venlafaxine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18207 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Anti-beta III Tubulin antibody - ab41489 from Abcam

Prices $390.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence - beta III Tubulin antibody (green) at a dilution of 1/1000; additional GFAP (red) and DAPI (blue) nuclear stain.

Anti-beta III Tubulin antibody - ab107216 from Abcam

Prices $385.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-beta III Tubulin antibody (ab107216) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryAnti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-beta III Tubulin antibody - ab76287 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
beta III Tubulin was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to beta III Tubulin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76287.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 50kDa; beta III Tubulin,non specific - as present in control (lane 2); We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest.

Anti-beta III Tubulin antibody - ab119100 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Immunohistochemical analysis of Neuron specific beta III Tubulin expression in formalin fixed, paraffin embedded Human Brain, Cerebellum tissue using ab119100 at 10 µg/ml.

Anti-beta III Tubulin antibody - ab76288 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-beta III Tubulin antibody (ab76288) at 1/1000 dilutionLane 1 : mouse brain lysateLane 2 : mouse brain lysate with unphosphorylated beta III Tublin (Ser 444) peptideLane 3 : mouse brain lysate with phospho beta III Tublin (Ser 444) peptide

Anti-beta III Tubulin antibody - ab66319 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-beta III Tubulin antibody (ab66319) at 1/500 dilution + normal human brain lysate at 10 µg

Anti-beta III Tubulin antibody - ab140861 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-beta III Tubulin antibody (ab140861) at 2 µg/ml + HepG2 cell line lysate (RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-beta III Tubulin antibody - ab117716 from Abcam

Prices $378.00
Sizes 250 µl
Host Chicken
Clonality Polyclonal
Immunohistochemistry Image of Formalin-Fixed, Paraffin-Embedded Mouse Brain stained with ab117716 at 5 µg/ml.

Anti-beta III Tubulin antibody - ab117740 from Abcam

Prices $378.00
Sizes 50 µl
Host Chicken
Clonality Polyclonal
ab117740, at 5 µg/ml, staining beta III Tubulin in Formalin-fixed, Paraffin-embedded Human Testis tissue by Immunohistochemistry.

Anti-beta III Tubulin antibody [2E9] - ab130209 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E9
All lanes : Anti-beta III Tubulin antibody [2E9] (ab130209) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : A549 cell lysateLane 3 : HeLa cell lysate

Anti-beta III Tubulin antibody [2E9] - ab91189 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2E9

Anti-beta III Tubulin antibody [2G10] - ab78078 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2G10
Immunohistochemistical detection of Neuron specific beta III Tubulin antibody using ab78078 on formaldehyde-fixed paraffin-embedded marmoset cerebellum sections. Antigen retrieval step: Heat mediated in Citric acid pH6 buffer. Permeabilization: No. Blocking step: 1% BSA for 10 mins @ rt°C. Primary Antibody used at 1/1000 incubated for 2 hours in TBS/BSA/azide. Secondary Antibody: anti mouse IgG conjugation to biotin (1/200).See Abreview

Anti-beta III Tubulin antibody [2G10] (FITC) - ab150202 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2G10

Anti-beta III Tubulin antibody [2G10] (HRP) - ab196638 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G10
All lanes : Anti-beta III Tubulin antibody [2G10] (HRP) (ab196638) at 1/5000 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissueLane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-beta III Tubulin antibody [5G8] - ab118627 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5G8
Overlay histogram showing HeLa cells stained with ab118627 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118627, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-beta III Tubulin antibody [5H2] - ab90202 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H2
Anti-beta III Tubulin antibody [5H2] (ab90202) at 1/2000 dilution + Lysate from 293T cells transfected with Human beta III Tubulin expression vector per lane at 5 µg

Anti-beta III Tubulin antibody [EP1331Y] - Microtubule Marker - ab52901 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1331Y
IHC-Fr image of Beta III Tubulin staining on zebrafish retina sections using ab52901 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab52901 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).See Abreview
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