Anti-beta III Tubulin antibody
| Name | Anti-beta III Tubulin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab107216 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Applications | IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human beta III Tubulin aa 350 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Chicken Polyclonal |
| Gene | TUBB3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-beta III Tubulin antibody (ab107216) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryAnti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab107216 stained U87-MG cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107216 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) U87-MG at 1ug/ml.
IHC-FoFr image of Neuron Specific beta III Tubulin staining on Mouse brain sections using ab107216 (1:3000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview
Product References
Congenitally acquired persistent lymphocytic choriomeningitis viral infection - Congenitally acquired persistent lymphocytic choriomeningitis viral infection
Sun T, Vasek MJ, Klein RS. PLoS One. 2014 May 6;9(5):e96442.
Clonal analysis reveals nerve-dependent and independent roles on mammalian hind - Clonal analysis reveals nerve-dependent and independent roles on mammalian hind
Rinkevich Y, Montoro DT, Muhonen E, Walmsley GG, Lo D, Hasegawa M, Januszyk M, Connolly AJ, Weissman IL, Longaker MT. Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9846-51. doi: