Anti-beta III Tubulin antibody [2G10] (HRP)

• Supplier: Abcam
• Catalog: ab196638
• Prices: $425.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 2G10
• Applications: IHC-P WB
• Species Reactivities: Mouse, Rat, Human, Bovine, Marmoset
• Antigen: Tissue, cells or virus corresponding to Rat beta III Tubulin
• Description: Mouse Monoclonal
• Gene: TUBB3
• Conjugate: HRP

Product images

All lanes : Anti-beta III Tubulin antibody [2G10] (HRP) (ab196638) at 1/5000 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissueLane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

IHC image of beta III Tubulin staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196638 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of beta III Tubulin staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196638 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.