Anti-beta III Tubulin antibody [EP1331Y] - Microtubule Marker

• Supplier: Abcam
• Catalog: ab52901
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1331Y
• Applications: WB IP ICC/IF FC IHC-P IHC-F
• Species Reactivities: Mouse, Rat, Human, Zebrafish
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
• Description: Rabbit Monoclonal
• Gene: TUBB3
• Conjugate: Unconjugated

Product images

IHC-Fr image of Beta III Tubulin staining on zebrafish retina sections using ab52901 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab52901 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).See Abreview

All lanes : Anti-beta III Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilutionLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Spinal Cord (Mouse) Tissue LysateLane 4 : Spinal Cord (Rat) Tissue LysateLane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Anti-beta III Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution + HeLa cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution

ab52901 at 1/250 dilution staining beta III Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.ab52901 at 1/250 dilution staining beta III Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.

ab52901 at 1/100 dilution staining beta III Tubulin in HeLa cells by Immunofluorescence.

Anti-beta III Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/1000 dilution (in PBS +0.5% Tween20 for 2 hours at 23°C) + 293 human embryonic kidney whole cell lysate at 25 µgSecondaryAn HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Overlay histogram showing HeLa cells stained with ab52901 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52901, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a diminished signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

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