Anti-beta IV Tubulin [EPR16775] antibody

• Supplier: Abcam
• Catalog: ab179504
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16775
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta IV Tubulin aa 50-150
• Description: Rabbit Monoclonal
• Gene: TUBB4A
• Conjugate: Unconjugated

Product images

All lanes : Anti-beta IV Tubulin [EPR16775] antibody (ab179504) at 1/5000 dilutionLane 1 : Human fetal brain lysatesLane 2 : Human cerebellum lysatesLane 3 : Human fetal kidney lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

All lanes : Anti-beta IV Tubulin [EPR16775] antibody (ab179504) at 1/5000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

All lanes : Anti-beta IV Tubulin [EPR16775] antibody (ab179504) at 1/5000 dilutionLane 1 : Mouse brain lysatesLane 2 : Mouse heart lysatesLane 3 : Mouse kidney lysatesLane 4 : Mouse spleen lysatesLane 5 : Rat brain lysatesLane 6 : Rat heart lysatesLane 7 : Rat kidney lysatesLane 8 : Rat spleen lysatesLane 9 : C6 (Rat glial tumor cells) whole cell lysatesLane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue). The  negative controls:--ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.-ve control 2 -  Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulin with ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).