TUBB4A

Gene Symbol	TUBB4A
Entrez Gene	10382
Alt Symbol	DYT4, TUBB4, beta-5
Species	Human
Gene Type	protein-coding
Description	tubulin, beta 4A class IVa
Other Description	dystonia 4, torsion (autosomal dominant)\|tubulin beta-4 chain\|tubulin beta-4A chain\|tubulin, beta 4\|tubulin, beta, 5
Swissprots	Q969E5 P04350 B3KQP4
Accessions	CAA25318 CAF86918 EAW69078 EAW69079 P04350 AK075307 BAG52106 AK095202 AK293503 BAG56988 AK294102 BAG57437 AK295917 BAG58705 BC006570 AAH06570 BC013683 AAH13683 DA265791 DA302559 DC303150 DC314277 DC353527 DC354036 EU831818 ACE87336 EU831895 ACE86654 NM_001289123 NP_001276052 NM_001289127 NP_001276056 NM_001289129 NP_001276058 NM_001289130 NP_001276059 NM_001289131 NP_001276060 NM_006087 NP_006078
Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subcellular Location	Cytoplasm, cytoskeleton.
Tissue Specificity	Major isotype in brain, where it represents 46% of all beta-tubulins. In the brain, highest expression levels in the cerebellum, followed by putamen and white matter. Moderate levels in testis. Very low levels, if any, in other tissues. {ECO:0000269\|PubMed:20191564, ECO:0000269\|PubMed:23424103}.
Top Pathways	Gap junction, Pathogenic Escherichia coli infection, Phagosome

β Tubulin (L-15) - sc-31782 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

β Tubulin (L-15): sc-31782. Western blot analysis of β Tubulin expression in non-transfected: sc-117752 (A) and human β2C Tubulin transfected: sc-114016 (B) 293T whole cell lysates.

Anti-beta IV Tubulin [EPR16775] antibody - ab179504 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR16775

All lanes : Anti-beta IV Tubulin [EPR16775] antibody (ab179504) at 1/5000 dilutionLane 1 : Human fetal brain lysatesLane 2 : Human cerebellum lysatesLane 3 : Human fetal kidney lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Anti-beta IV Tubulin antibody - ab104310 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-beta IV Tubulin antibody (ab104310) at 1 µg/ml + Jurkat cell lysate at 10 µg

Anti-beta IV Tubulin antibody [6E3] - ab119254 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	6E3

All lanes : Anti-beta IV Tubulin antibody [6E3] (ab119254) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY beta IV TubulinLysates/proteins at 5 µg per lane.

Anti-beta IV Tubulin antibody [ONS.1A6] - ab11315 from Abcam

Prices	$390.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	ONS.1A6

All lanes : Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315) at 5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 6 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-TUBB4 Antibody (clone 5C1) - LS-C173086 from LifeSpan Bioscience

Prices	$285.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	5C1

IHC of paraffin-embedded Human Kidney tissue using anti-TUBB4 mouse monoclonal antibody.

Anti-TUBB4 Antibody (clone 3F1) - LS-C172999 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3F1

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TUBB4 mouse monoclonal antibody.

Anti-TUBB4 Antibody (clone 6C2) - LS-C173057 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	6C2

IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TUBB4 mouse monoclonal antibody.

Anti-TUBB4 Antibody (clone 3C12) - LS-C172984 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3C12

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBB4 mouse monoclonal antibody.

Anti-TUBB4 Antibody (C-Terminus) - LS-C92886 from LifeSpan Bioscience

Prices	$485.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal

Anti-TUBB4 Antibody (aa35-84) - LS-C116447 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TUBB4 antibody LS-C116447 Western blot of Jurkat lysate.

Anti-TUBB4 Antibody (clone 6E3) - LS-C173087 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	6E3

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB4.

Anti-TUBB4 Antibody (clone 8E3) - LS-C173072 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	8E3

Anti-TUBB4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB4.

Tubulin Beta 4 Antibody (3F1) - NBP2-00812 from Novus Biologicals

Prices	$379.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3F1

Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin Beta 4 monoclonal antibody.

Tubulin Beta 4 Antibody - NBP1-32205 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample(30 ug of whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:1000.

Tubulin Beta 4 Antibody - NBP1-57005 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Tubulin Beta 4 Antibody (ONS.1A6) - NB120-11315 from Novus Biologicals

Prices	$279.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	ONS.1A6

Western Blot: Tubulin Beta 4 Antibody (ONS.1A6) [NB120-11315] - MCF7 Human breast carcinoma cells extract was separated on SDS-PAGE

Anti-TUBB4 (154-164) antibody produced in rabbit - SAB1104795 from Sigma-Aldrich

Prices	$285.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells was separated on SDS-PAGE, blotted with Anti-TUBB4 (154-164) (<B>Cat. No. SAB1104795</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Anti-TUBB4, (N-terminal) antibody produced in rabbit - SAB2105810 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-TUBB4 (N-Terminal): <B>Cat. No. SAB2105810</B>: TUBB4 in Jurkat cell lysates with TUBB4 antibody at 1.0 μg/mL.

Monoclonal Anti-β-Tubulin IV antibody produced in mouse - T7941 from Sigma-Aldrich

Prices	$340.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Clone	ONS.1A6

<B>Immunoblotting</B><BR/>MCF7 Human breast carcinoma cells extract was separated on SDS-PAGE and probed with Monoclonal AB To β Tubulin IV CL:ONS.1A6 (<B>Cat. No. T7941</B>). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (<B>Cat. No. A1293</B>) and a NBT/BCIP substrate.<BR/>Antibody dilution 1/400.

TUBB4 antibody - N-terminal region (ARP52132_P050) - ARP52132_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

TBB4A Antibody - C-terminal region (ARP75349_P050) - ARP75349_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

beta Tubulin 3/ Tuj1 antibody - GTX85469 from GeneTex

Prices	$289.00
Sizes	50 μg
Host	Chicken
Clonality	Polyclonal

Anti-beta III Tubulin antibody used in Immunohistochemistry. GTX85469

beta Tubulin 3/ Tuj1 antibody [5H2] - GTX83460 from GeneTex

Prices	$369.00
Sizes	100 μl
Host	Mouse
Clonality	Monoclonal
Clone	5H2

Anti-beta III Tubulin antibody [5H2] used in Western blot. GTX83460

TUBB3 antibody - 10R-6182 from Fitzgerald

Prices	$455.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	5H2

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