All lanes : Anti-beta IV Tubulin antibody [6E3] (ab119254) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY beta IV TubulinLysates/proteins at 5 µg per lane.
Flow cytometric analysis of HEK293T cells transfected with either beta IV Tubulin overexpress plasmid (Red) or empty vector control plasmid (Blue), immunostained with ab119254 at a 1/100 dilution.
Flow cytometric analysis of HeLa cells immunostained with ab119254 at a 1/100 dilution (Red) or a nonspecific negative control antibody,(Blue).
Flow cytometric analysis of Jurkat cells immunostained with ab119254 at a 1/100 dilution (red) or a nonspecific negative control antibody,(Blue).