Anti-beta Synuclein antibody

• Supplier: Abcam
• Catalog: ab6165
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF IHC-P IHC-F WB
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide
• Description: Rabbit Polyclonal
• Gene: SNCB
• Conjugate: Unconjugated

Product images

ab6165 staining beta synuclein in human occipital cortex.Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Product References

The decrease of beta-synuclein in cortical brain areas defines a molecular - The decrease of beta-synuclein in cortical brain areas defines a molecular

Beyer K, Domingo-Sabat M, Santos C, Tolosa E, Ferrer I, Ariza A. Brain. 2010 Dec;133(Pt 12):3724-33.

alpha-Synuclein affects the MAPK pathway and accelerates cell death. - alpha-Synuclein affects the MAPK pathway and accelerates cell death.

Iwata A, Maruyama M, Kanazawa I, Nukina N. J Biol Chem. 2001 Nov 30;276(48):45320-9. Epub 2001 Sep 17.