Anti-beta Tubulin antibody

• Supplier: Abcam
• Catalog: ab15568
• Prices: $403.00
• Sizes: 500 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: FC ICC/IF ICC/IF IHC-P IP WB
• Species Reactivities: Mouse, Bovine, Human, Rat, Chicken, Guinea Pig, Dog, Pig, Yeast, Xenopus, Chimpanzee, Sea Urchin, Fungi, Xenopus
• Antigen: Synthetic peptide: NDLVSEYQQYQDATA , corresponding to amino acids 416-430 of Human beta Tubulin
• Description: Rabbit Polyclonal
• Gene: TUBB
• Conjugate: Unconjugated

Product images

All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).See Abreview

ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).

Product References

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