TUBB

Gene Symbol TUBB
Entrez Gene 396254
Alt Symbol TUBB2A
Species Chicken
Gene Type protein-coding
Description tubulin, beta class I
Other Description beta 5-tubulin|c(beta)7 tubulin|tubulin beta 4'|tubulin beta-7 chain|tubulin, beta 2A|tubulin, beta polypeptide
Swissprots P09244
Accessions P09244 X07011 CAA30060 NM_205315 NP_990646
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subcellular Location Cytoplasm, cytoskeleton.
Top Pathways Gap junction, Phagosome

Anti-beta Tubulin antibody - ab15568 from Abcam

Prices $403.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-beta Tubulin antibody - ab21057 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : HeLa whole cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryRabbit anti Goat polyclonal at 1/10000 dilution

Anti-beta Tubulin antibody - ab140698 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney (glomeruli) tissue labelling beta Tubulin with ab140698 at 10 µg/ml.

Anti-beta Tubulin antibody - ab125285 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab125285, at 1/200 dilution, staining beta Tubulin in paraffin-embedded Human lung tissue by Immunohistochemistry.

Anti-beta Tubulin antibody - ab18587 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-beta Tubulin antibody [2-28-33] - ab11311 from Abcam

Prices $378.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 2-28-33
ab11311 staining beta tubulin in primary human keratinocytes by Immunocytochemistry/ Immunofluorescence.Cells treated with 0.1% DMSO for 18 hours.

Anti-beta Tubulin antibody [BT7R] - ab135209 from Abcam

Prices $378.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone BT7R
Anti-beta Tubulin antibody [BT7R] (ab135209) at 1/2000 dilution + Mouse brain tissue lysates at 10 µg

Anti-beta Tubulin antibody [BT7R] - ab169832 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BT7R

Anti-beta Tubulin antibody [BT7R] (Biotin) - ab173839 from Abcam

Prices $384.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone BT7R
Immunofluorescent analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody (ab173839) at a dilution of 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken  at 40X magnification.

Anti-beta Tubulin antibody [BT7R] (HRP) - ab173840 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone BT7R
All lanes : Anti-beta Tubulin antibody [BT7R] (HRP) (ab173840) at 1/1000 dilutionLane 1 : A431 cell lysateLane 2 : U2OS cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLane 5 : HeLa cell lysateLane 6 : COS7 cell lysateLane 7 : C2C12 cell lysateLane 8 : NRK cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-beta Tubulin antibody [D66] - ab11307 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone D66

Anti-beta Tubulin antibody [EPR16774] - ab179513 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16774
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/1000 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).The negative controls are as follows;-ve control 1: ab179513 at 1/1000 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. 

Anti-beta Tubulin antibody, prediluted - ab15569 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15569 staining beta Tubulin in human lung by Immunohistochemistry (FFPE-sections).

Anti-beta Tubulin antibody [TBN06] - ab150754 from Abcam

Prices $378.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone TBN06
Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung tissue labeling beta Tubulin with ab150754 at 2 µg/ml for 30 minutes at room temperature.

Anti-beta Tubulin antibody [TU-06] - ab7792 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone TU-06
Anti-beta Tubulin antibody [TU-06] (ab7792) at 1/500 dilution + Human U87 MG whole cell lysate with 5% Milk for 1 hour at 22°C.SecondaryHRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-beta Tubulin antibody - Loading Control - ab6046 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
The cells were 100% methanol fixed (5 min) and thenincubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) and (ab37266, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green LCS1 (ab138904) was used to stain the cell nuclei (green) at a dilution of 1/500. 

Anti-beta Tubulin antibody - Loading Control (HRP) - ab21058 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-beta Tubulin antibody - Loading Control (HRP) (ab21058) at 1/1000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Tubulin antibody - ab11270 from Abcam

Prices $372.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Tubulin antibody [B3] - ab11324 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone B3

Anti-Tubulin antibody [DM1A +DM1B] - ab44928 from Abcam

Prices $397.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DM1A +DM1B
Ab44928 staining human normal placenta. Staining is localized to the cytoplamsmLeft panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Tubulin monoclonal antibody, clone 656 - DMAB6589 from Creative Diagnostics

Host Mouse
Clonality Monoclonal
Clone 656

TUBB2C antibody - orb35423 from Biorbyt

Prices $187.00, $383.00
Sizes 80 μl (Trial size), 400 μl
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of paraffin embedded human brain tissue using TUBB2C antibody (primary antibody dilution at: 1:50-100)
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