Anti-beta Tubulin antibody [EPR16774]

• Supplier: Abcam
• Catalog: ab179513
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16774
• Applications: IHC-P WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Chicken, Bovine, Dog, Human, Drosophila, Monkey, Zebrafish, Xenopus
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta Tubulin aa 400 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: TUBB
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/1000 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).The negative controls are as follows;-ve control 1: ab179513 at 1/1000 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. 

Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/1000 dilution + Human beta I Tubulin recombinant protein at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilutionLane 1 : Zebrafish whole lysatesLane 2 : Xenopus tropicalis lysatesLane 3 : Drosophila whole lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilutionLane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysatesLane 2 : MDCK (Canine kidney cell line) whole cell lysatesLane 3 : MDBK (Bovine kidney cell line) whole cell lysatesLane 4 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLane 3 : A431 (Human epidermoid carcinoma) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilutionLane 1 : Human fetal brain lysatesLane 2 : Human fetal kidney lysatesLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilutionLane 1 : Mouse brain lysatesLane 2 : Rat brain lysatesLane 3 : C6 (Rat glial tumor cells) whole cell lysatesLane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on tubules and the glomerulus of human kidney is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on human glioma is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat cerebral cortex is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.