Anti-beta Tubulin antibody [TU-06]
| Name | Anti-beta Tubulin antibody [TU-06] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7792 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | TU-06 |
| Applications | ELISA WB ICC/IF FC |
| Species Reactivities | Mouse, Human, Pig, Zebrafish, Rat, Horse, Chicken, Hamster, Bovine, Dog, Plant, Monkey, Monkey, Ape, Xenopus |
| Antigen | Beta-subunits of tubulin (purified) (Pig) |
| Description | Mouse Monoclonal |
| Gene | TUBB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-beta Tubulin antibody [TU-06] (ab7792) at 1/500 dilution + Human U87 MG whole cell lysate with 5% Milk for 1 hour at 22°C.SecondaryHRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/14298_beta-Tubulin-Primary-antibodies-ab7792-2.jpg)
Anti-beta Tubulin antibody [TU-06] (ab7792) at 1/500 dilution + Human U87 MG whole cell lysate with 5% Milk for 1 hour at 22°C.SecondaryHRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview
![All lanes : Anti-beta Tubulin antibody [TU-06] (ab7792) at 1/2000 dilutionLane 1 : Mouse liver tissue lysateLane 2 : Mouse hepatocyte cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 52 kDa (why is the actual band size different from the predicted?)Exposure time : 10 secondsThis image is courtesy of an anonymous AbreviewSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/14299_ab7792-237755-7792wb.jpg)
All lanes : Anti-beta Tubulin antibody [TU-06] (ab7792) at 1/2000 dilutionLane 1 : Mouse liver tissue lysateLane 2 : Mouse hepatocyte cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 52 kDa (why is the actual band size different from the predicted?)Exposure time : 10 secondsThis image is courtesy of an anonymous AbreviewSee Abreview
ICC/IF image of ab7792 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7792, 1 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.
![Overlay histogram showing HeLa cells stained with ab7792 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7792, 1µg/1x10^6 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100® used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/14301_ab7792-194549-ab77922.jpg)
Overlay histogram showing HeLa cells stained with ab7792 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7792, 1µg/1x10^6 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100® used under the same conditions.
Product References
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