All lanes : Anti-Bid antibody (ab63541) at 1/500 dilutionLane 1 : Extracts from Jurkat cells (5ug) treated with H2O2 (100uM, 30mins)Lane 2 : Extracts from Jurkat cells (5ug) treated with etoposide (25uM, 60 mins)Lane 3 : Extracts from Jurkat cells (5ug) treated with H2O2 (100uM, 30mins) and etoposide (25uM, 60 mins) with immunizing non-phosphopeptide at 5 µg
Ab63541 (1:50) staining human Bid in human brain tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue. The left hand panel is without the presence of blocking peptide, whereas the right hand panel is in the presence of blocking peptide.