All lanes : Anti-Bid antibody [2H9] (ab180394) at 1/400 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Bid cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-Bid antibody [2H9] (ab180394) at 1/400 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : mouse SVT2 cell lysateLane 4 : A549 cell lysateLane 5 : monkey COS7 cell lysateLane 6 : Jurkat cell lysateLane 7 : canine MDCK cell lysateLane 8 : rat PC12 cell lysateLane 9 : MCF7 cell lysateLysates/proteins at 35 µg per lane.
Immunofluorescence analysis of monkey COS7 cells transiently transfected with Bid, labeling Bid using ab180394 at a 1/100 dilution