Anti-Bik antibody
| Name | Anti-Bik antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab52182 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic non-phosphopeptide (human) around the phosphorylation site of threonine 33 (G-M-T-D-S) |
| Description | Rabbit Polyclonal |
| Gene | BIK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Bik antibody (ab52182) at 1/500 dilutionLane 1 : extracts from A549 cells, treated with DMSO (0.1%, 10mins)Lane 2 : extracts from A549 cells, treated with DMSO (0.1%, 10mins) with blocking peptide
Ab52182 at 1/50 dilution staining Human breast carcinoma tissue, with and without blocking peptide; paraffin embedded.
ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.See Abreview