Flow Cytometry analysis of C2C12 cells labeling BIN1 with ab181710 and control (Anti-IgD) antibody. Secondary antibody: Biotin mouse secondary antibody at 1/10,000 for 45 min at RT and streptavidin PE at 1/5,000 for 30 min at RT.
All lanes : Anti-BIN1 antibody [99D] (ab181710) at 1/5000 dilutionLane 1 : Lysate of Keratinocyte derived from BIN1 wild type mice.Lane 2 : Lysate of Keratinocyte derived from BIN1 null type mice.Lysates/proteins at 35 µg per lane.SecondaryAnti-mouse secondary antibody at 1/10000 dilutiondeveloped using the ECL technique