
All lanes : Anti-BIN2 antibody - N-terminal (ab175482) at 1/1000 dilutionLane 1 : HL60 cell line lysateLane 2 : CEM cell line lysateLane 3 : MDA-MB435 cell line lysateLane 4 : A549 cell line lysateLysates/proteins at 15 µg per lane.

Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling BIN2 with ab175482 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow Cytometrical analysis of CEM cells labeling BIN2 with ab175482 antibody at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.