Anti-BIRC6 antibody

• Supplier: Abcam
• Catalog: ab19609
• Prices: $370.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP IHC-P
• Species Reactivities: Human, Mouse, Chimpanzee, Monkey, Orangutan
• Antigen: The epitope recognized maps to a region between residue 4775 and the C-terminus (residue 4829) of human Baculoviral IAP repeat-containing protein 6 using the numbering given in Swiss-Prot entry Q9NR09
• Description: Rabbit Polyclonal
• Gene: BIRC6
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BIRC6 with ab19609 at 1/5000 (0.2µg/ml). Detection: DAB.

All lanes : Anti-BIRC6 antibody (ab19609) at 1/10000 dilutionLane 1 : HeLa whole cell lysate at 20 µgLane 2 : HeLa whole cell lysate at 50 µgLane 3 : HeLa S100 extract at 20 µgLane 4 : HeLa S100 extract at 50 µg

Detection of human BIRC6 by Immunoprecipitation. Affinity purified ab19609 used at 2mcg/mg lysate (whole cell lysate (10mg) from HeLa cells. The immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6 was confirmed by mass spectrometry.

ICC/IF image of ab19609 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19609, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

gamma-Synuclein antibodies have neuroprotective potential on neuroretinal cells - gamma-Synuclein antibodies have neuroprotective potential on neuroretinal cells

Wilding C, Bell K, Beck S, Funke S, Pfeiffer N, Grus FH. PLoS One. 2014 Mar 3;9(3):e90737.

Identification of BIRC6 as a novel intervention target for neuroblastoma therapy. - Identification of BIRC6 as a novel intervention target for neuroblastoma therapy.

Lamers F, Schild L, Koster J, Speleman F, Ora I, Westerhout EM, van Sluis P, Versteeg R, Caron HN, Molenaar JJ. BMC Cancer. 2012 Jul 12;12:285.

Comparative proteomics of colon cancer stem cells and differentiated tumor cells - Comparative proteomics of colon cancer stem cells and differentiated tumor cells

Van Houdt WJ, Emmink BL, Pham TV, Piersma SR, Verheem A, Vries RG, Fratantoni SA, Pronk A, Clevers H, Borel Rinkes IH, Jimenez CR, Kranenburg O. Mol Cell Proteomics. 2011 Dec;10(12):M111.011353.

Apollon gene silencing induces apoptosis in breast cancer cells through p53 - Apollon gene silencing induces apoptosis in breast cancer cells through p53

Lopergolo A, Pennati M, Gandellini P, Orlotti NI, Poma P, Daidone MG, Folini M, Zaffaroni N. Br J Cancer. 2009 Mar 10;100(5):739-46.