Anti-BMPR1A antibody
| Name | Anti-BMPR1A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38560 |
| Prices | $385.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human BMPR1A aa 166-195 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | BMPR1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical (formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling BMPR1A with ab38560. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
All lanes : Anti-BMPR1A antibody (ab38560) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysate SecondaryGoat anti-rabbit (H+L) at 1/5000 dilution
ICC/IF image of ab38560 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
BMPRIA mediated signaling is essential for temporomandibular joint development in - BMPRIA mediated signaling is essential for temporomandibular joint development in
Gu S, Wu W, Liu C, Yang L, Sun C, Ye W, Li X, Chen J, Long F, Chen Y. PLoS One. 2014 Aug 5;9(8):e101000.
Reduced expression of bone morphogenetic protein receptor IA in pancreatic cancer - Reduced expression of bone morphogenetic protein receptor IA in pancreatic cancer
Voorneveld PW, Stache V, Jacobs RJ, Smolders E, Sitters AI, Liesker A, Korkmaz KS, Lam SM, De Miranda NF, Morreau H, Kodach LL, Hardwick JC. Br J Cancer. 2013 Oct 1;109(7):1805-12.