![Overlay histogram showing HepG2 cells stained with ab130206 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130206, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/15062_ab130206-6-ab130206FC.jpg)
Overlay histogram showing HepG2 cells stained with ab130206 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130206, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab130206 at 1/200 staining BMPR2 in paraffin-embedded tonsil tissue by immunohistochemistry with DAB staining.
![All lanes : Anti-BMPR2 antibody [1F12] (ab130206) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : NIH/3T3 cell lysateLane 3 : Cos7 cell lysateLane 4 : PC12 cell lysateLane 5 : Cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/15064_BMPR2-Primary-antibodies-ab130206-6.jpg)
All lanes : Anti-BMPR2 antibody [1F12] (ab130206) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : NIH/3T3 cell lysateLane 3 : Cos7 cell lysateLane 4 : PC12 cell lysateLane 5 : Cell lysate