Anti-BMX antibody
| Name | Anti-BMX antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59288 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic non-phosphopeptide derived from human ETK around the phosphorylation site of tyrosine 40 (L-S-YP-Y-E) |
| Description | Rabbit Polyclonal |
| Gene | BMX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab59288 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59288 at 5 µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab59288 at 1/50 dilution staining human skin tissue without (left) and with (right) immunizing peptide; paraffin embedded.Ab59288 at 1/50 dilution staining human skin tissue without (left) and with (right) immunizing peptide; paraffin embedded.