Anti-Borealin/CDCA8 antibody
| Name | Anti-Borealin/CDCA8 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab67126 |
| Prices | $380.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Full length native Borealin/CDCA8 (purified) (Human) |
| Description | Mouse Polyclonal |
| Gene | CDCA8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilutionLane 1 : Borealin/CDCA8 transfected 293T cell lysateLane 2 : Untransfected 293T cell lysateSecondaryGoat anti mouse IgG (H&L)-HRP conjugate secondary antibody at 1/2500 dilution
ab67126 staining Borealin/CDCA8 in HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 10% BSA for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 23°C. The secondary antibody used was a mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/300 dilution.When fixed in PFA, the antibody stains the centromere in metaphase and the midzone in anaphase, as expected for Borealin, a CPC protein. Borealin (red), Kinetochores (green), DAPI (blue).See Abreview